MICROBIOLOGYDNA FLOW technology

The target DNA molecules cross the membrane through a vertical vacuum flow, facilitating their interaction with the specific and complementary DNA probes immobilised on the porous membrane in a three-dimensional environment, unlike conventional dot blot technology in which hybridisation occurs passively. This accelerates the process and increases sensitivity, reducing reaction volumes and overall processing time from several hours to a few minutes.

Once binding has occurred between the specific amplicons and their corresponding probes, this is visualised by a colorimetric immunoenzymatic reaction with Streptavidin-Alkaline Phosphatase and the BCIP/NBT chromogen, forming insoluble precipitates on the membrane in those areas where hybridisation has occurred. The process is performed automatically in the HybriSpot™ platform and the results are analysed with the HybriSoft™ software.